Development Background
In routine microbial experiments,whether it is food hygiene bacteriological testing,drug microbial limit inspection,or research on the antibacterial properties of active substances,it is often necessary to quantify or calculate the concentration of microorganisms in the sample.The most commonly used method of microbial quantification in China is the statistical quantification method of the total number of colonies grown on the cultured Petri dish.
The statistical and quantitative method of the total number of colonies is also the legal standard method for the quantitative inspection of microorganisms in most national standards and the legal reference method for other quantitative methods of microorganisms.
traditional counting method
At present,most laboratories still use the traditional method of counting with the human eye combined with a colony counter:using a magnifying glass,click each colony with a counting pen,and the counter counts the pressure electronic pulse generated by each click to obtain the total number.Although this is cheap,it also has many disadvantages:
1.Identify and count errors and omissions
All counts rely on the pressure to record the number of pressure pulses.Different pressures generated by different operators will inevitably lead to missed records,and the results of the counts are also different due to the operator's different experience in colony identification,and the system deviation is large..Many laboratories"return"to purely manual visual counting after using colony counter-assisted counting for a period of time.
2.Marking and Correcting Inconvenience
Both visual counting and colony counter counting rely on the counting pen to leave ink marks on the counted colonies,so it is inconvenient to mark when there are dense colonies,and it is necessary to wipe off the ink marks when miscounting is found,which leads to inconvenience of correction.
3.Inefficiency
Both naked eye counting and colony counter counting require manual identification of each colony with the naked eye,so once the number of samples is large,it will consume a lot of time and affect the efficiency of research or result reporting.
4.The original result cannot be saved
After the statistics are done,a statistic is left,and the colony growth status on the surface of the culture plate,which is important for recording and verifying the experimental results,cannot be saved because the plate is washed off.
As the most common monitoring item in microbial testing,the total number of colonies should be carefully and precisely operated in every link of the test to ensure the accuracy of the test results.Nowadays,the emergence of automatic colony counter will gradually replace the traditional manual visual counting method,greatly save time,improve accuracy,realize digital management,and create a digital era of microbial colony detection.
Automatic Colony Counter
The automatic colony counter of Shanghai Haicoer artificial intelligence is composed of three parts:image input,image processing software and data storage.The software system collects digital images of the cultured colonies through the image input device,transmits them to the computer terminal for counting receipts through the USB port data communication,performs a series of image algorithm processing on the input digital images,outputs the counting results,and performs manual correction according to the situation.,after the counting is completed,the relevant files can be saved,and the output data can be stored in the computer.
Product advantages
1.More efficient
It can quickly photograph,identify and calculate the colony culture dishes,and the detection speed is as high as 4 seconds per piece.Save more time for inspectors and increase inspection efficiency tenfold.
2.Safer
Reduce the eye time of the inspectors,improve the working environment,and reduce the work intensity.
3.Save more
The traditional detection mode of"eyes+brain"has been changed,and the efficient detection saves manpower and reduces labor costs by 50%.
Shanghai Haicoer automatic colony counter is tailor-made for microbiology laboratory users,with a high degree of automation,accurate counting,analysis results can be checked,and sample information can be retained,which can save a lot of manpower and time.Make your work easier and more efficient.
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